α-Naphthyl Butyrate Esterase (α-NBE) Stain
Intended Use:
This kit is for staining bone marrow cell and bloodcell smear.
Principle:
Under alkaline condition, α-Naphthyl Butyrate is hydrolyzed into α-naphthol by Esterase. α-naphthol shall combine with diazonium salt to forminsoluble colored precipitate in cytoplasm. This stain has no specificity against esterase; therefore, it is called"Non-specific Esterase Stain".
Specifications:
|
Contents |
5Tests/Kit |
20Tests/Kit |
100Tests/Kit |
Components |
|
Fixative |
1vial x 5ml |
1vial x 20ml |
1vial x 100ml |
Sodium Citrate |
|
Pararosaniline |
1vial x 0.1ml |
1vial x 0.3ml |
1vial x 1.1ml |
Parafuchsin |
|
Sodium Nitrite |
1vial x 0.1ml |
1vial x 0.3ml |
1vial x 1.1ml |
Sodium Nitrite |
|
Phosphate Buffer |
1vial x 25ml |
1vial x 90ml |
2vials x 200ml |
Phosphate |
|
α-Naphthyl Butyrate |
1vial x 1ml |
1vial x 4.5ml |
1vial x 21ml |
α-Naphthyl |
|
Methyl Green |
1vial x 5ml |
1vial x 20ml |
1vial x 100ml |
Methyl Green |
Methods:
Working solution Preparation (for 1 test only):
Apparatus required: disposable tube, micropipette, disposable tips, dropper;
Instruction: Add 10µl solution B to 10µl solution C, mix well and wait for 1 minute;
Add 4ml solution D and 200µl solution E. Mix well (Add 2 drops of solution NaFfor NaF inhibition test.)
|
Solution B |
Solution C |
Solution D |
Solution E |
Solution |
|
|
Tube Α |
10µl |
10µl |
4ml |
200µl |
- |
|
Tube B |
10µl |
10µl |
4ml |
200µl |
2drops |
Expected Results:
Red or red-brown granules found in cytoplasm means positive.
