Naphthol AS-D Chloroacetate Esterase (AS–DCE) Stain

Intended Use:

This kit is for staining bone marrow cell and bloodcell smear.

 

Principle:

Naphthol AS-D chloroacetate can be hydrolyzed into  Naphthol AS-D by Esterase to mix with diazonium salt and form insoluble red-brown precipitate in cytoplasm. Positive reaction is only found in granulocytes; hence, it is also called "Specific Esterase Stain".

 

Specifications:

 

Contents	5Tests/Kit	20Tests/Kit	100Tests/Kit	Components
Fixative (solution A)	1vial×2.5m	1vial×10ml	1vial×50ml	Sodium Citrate
Pararosaniline (solution B)	1vial×0.1ml	1vial×0.3ml	1vial×1.1ml	Parafuchsin
Sodium Nitrite (solution C)	1vial×0.1ml	1vial×0.3ml	1vial×1.1ml	Sodium Nitrite
Phosphate Buffer (solution D)	1vial×5ml	1vial×20ml	1vial×95ml	Phosphate
Naphthol As-D Chloroacetate (solution E)	1vial×0.3ml	1vial×1.2ml	1vial×5.5ml	Naphthol AS-D hloroacetate
Methyl Green (solution F)	1vial×5ml	1vial×20ml	1vial×100ml	Methyl Green

 

Methods:

Working solution Preparation（for 1 test only）;

Apparatus required: disposable tube, micropipette, disposable tips, dropper;

Instruction: Mix 10µl solution B and10µl solution C thoroughly. Wait for 1 Minute;

Add 0.9ml solution D and 50µl solution E. Mix well;

 

Expected Results:

Red granules found in cytoplasm means positive.