Neutrophilic- Leukocyte Alkaline Phosphatase (NAP) Stain

 

Intended Use:

This kit is for staining bone marrow cell and bloodcell smear.

 

Principle:

Neutrophilic-Leukocyte Alkaline Phosphatase stain is based on diazotization-coupling principle. At pH9.2~9.8, Alkaline Phosphatase hydrolyzes Naphthol AS-BI Phosphoric Acid into  α-naphthol. The stable diazonium salt and naphthol shall form insoluble colored precipitate in cytoplasm.

 

Specifications:

Contents	5Tests/Kit	20Tests/Kit	100Tests/Kit	Components
Fixative (solution A)	1vial×5ml	1vial×20ml	1vial×100ml	Sodium Citrate
FBB -Alkaline (solution B)	1vial×0.3ml	1vial×1.1ml	1vial×5.1ml	Fast Blue BB Base
Sodium Nitrite (solution C)	1vial×0.3ml	1vial×1.1ml	1vial×5.1ml	Sodium Nitrite
Naphthol AS-Bl Alkaline (solution D)	1vial×0.3ml	1vial×1.1ml	1vial×5.1ml	Naphthol AS-Bl
Neutral Red  (solution E)	1vial×5ml	1vial×20ml	1vial×100ml	Neutral Red

 

Methods:

Working solution Preparation (for 1 test only)

Apparatus required：disposable tube, micropipette, disposable tips, dropper;

Instruction：Mix 25µl solution B with 25µl C; wait for 2 minutes. Add distilled water 2ml into 25µl solution D. Mix thoroughly.

 

Expected Results:

NΑP positive granules are blue.